Abstract
An assay has been developed for the colorimetric estimation of total amino acids in small volumes (100 μl) of whole blood. The procedure utilizes the reaction of amino nitrogen with β-naphthoquinone sulfonate. It represents a substantial improvement on previously published methods by decreasing reagent volumes and accurately specifying and maintaining pH conditions for the reaction itself (9.2-9.4) and for removal of excess reagent prior to measurement of the resulting complex (2.2-2.4). Ammonia is the only compound shown to significantly interfere with the assay but this is not a problem at physiological concentrations.
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