Abstract
The development of probes for rapid, selective, and sensitive detection of the hydrogen polysulfides is of great importance in biological science. We report here a new Near-infrared (NIR) fluorescent off-on probe for selective and sensitive detection of hydrogen polysulfides (H2Sn) with colorimetric and NIR fluorescent dual signal changes. The probe was synthesized by an esterification reaction coupling between a dicyanomethylene-benzopyran (DCMB) dye and a bis-electrophilic H2Sn capture group 2-fluoro-5-nitrobenzoic acid. The probe showed a specific off-on response to H2Sn in aqueous solution with nanomolar LOD, and without interference by a range of competing sulfocompounds and several reactive nitrogen and oxygen species. Moreover, it can be used for imaging of endogenous H2Sn in living cells.
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