Abstract
The growing applications of tissue engineering technologies warrant the search and development of biocompatible materials with an appropriate strength and elastic moduli. Here, we have extensively studied a collagenous membrane (GSCM) separated from the mantle of the Giant squid Dosidicus Gigas in order to test its potential applicability in regenerative medicine. To establish the composition and structure of the studied material, we analyzed the GSCM by a variety of techniques, including amino acid analysis, SDS-PAGE, and FTIR. It has been shown that collagen is a main component of the GSCM. The morphology study by different microscopic techniques from nano- to microscale revealed a peculiar packing of collagen fibers forming laminae oriented at 60–90 degrees in respect to each other, which, in turn, formed layers with the thickness of several microns (a basketweave motif). The macro- and micromechanical studies showed high values of the Young’s modulus and tensile strength. No significant cytotoxicity of the studied material was found by the cytotoxicity assay. Thus, the GSCM consists of a reinforced collagen network, has high mechanical characteristics, and is non-toxic, which makes it a good candidate for the creation of a scaffold material for tissue engineering.
Highlights
The basic objective of tissue engineering consists of obtaining such a scaffold material that would promote complete or at least partial regeneration of internal organs, skin, vascular, bone, cartilage, and other tissues
The squid mantle consists of several layers (Figure 15), with the central muscle layer surrounded by two collagenous membranes; the GSCM represents the outer tunic of the mantle
The literature analysis shows that the GSCM material has been very poorly studied, and no application in tissue engineering has been discussed so far
Summary
The basic objective of tissue engineering consists of obtaining such a scaffold material that would promote complete or at least partial regeneration of internal organs, skin, vascular, bone, cartilage, and other tissues. The construct’s biocompatibility, biodegradability, as well as mechanical properties determine its potential to substitute the corresponding live tissue in the body. The interest to collagen-based materials is stipulated by the fact that collagen is biocompatible with the recipients’ tissues, can biodegrade, is non-toxic, non-carcinogenic and non-immunogenic, and combines many characteristics of synthetic polymers (strength, stiffness, ability to form various supramolecular structures, etc.). A plethora of pharmaceutical preparations and medical devices have been created using collagen as a base [1]. Collagen is one of basic natural materials which have application in tissue engineering [2–6]. In many types of connective tissue, it is a fibrillar protein and the main component responsible for the tissue integrity, shape, elasticity, and strength
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
