Abstract
Abstract The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35 °C. The endoglucanase activity, melt point temperature was 1.13 folds (247.02 U·ml− 1), 2.1 °C higher (39.2 °C) in 6% ethanol solution than that (218.60 U·ml− 1), (37.1 °C) in free ethanol. At 40 °C–55 °C, Gibbs free energy, ΔG, and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.
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