Abstract
Application of the amino acid analyzer is described for the separation, identification, and estimation of several uncommon dibasic amino acids that are eluted with a 0.35 m, pH 5.28, sodium citrate buffer between lysine and ammonia, namely, 2,5-diaminohexanoate, threo- and erythro-3,5-diaminohexanoate, 3,6-diaminohexanoate (β-lysine) and 2,4-diaminopentanoic acid. Conditions are also given for estimating d- and l-β-lysine as their l-glutamyl peptides, and for estimating the enantiomers of threo- and erythro-3,5-diaminohexanoate as the l-glutamyl derivative of the corresponding lactams.
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