Abstract
A simple and efficient cloud point extraction-spectrofluorimetric method for the determination of thiamine in human urine is proposed. The procedure is based on the oxidation of thiamine with ferricyanide to form thiochrome, its extraction to Triton X-114 micelles and spectrofluorimetric determination. The variables affecting oxidation of thiamine, extraction and phase separation were studied and optimized. Under the experimental conditions used, the calibration graphs were linear over the range 2.5-1000 ng . The limit of detection was 0.78 ng of thiamine and the relative standard deviation for 5 replicate determinations of thiamine at 400 ng concentration level was 2.42%. Average recoveries between 93-107% were obtained for spiked samples. The proposed method was applied to the determination of thiamine in human urine.ꤠ돀遠�⨀堘�⨀袒�⨀脠돐ꢒ�⨀ࢷ�⨀낒�⨀夠뎐／ࠀ⣕�⨀愁덀态Ȁ胮傫i傫Āā?뤞덐塲�⨀퀿ﶖ⨀⩈ﶖ⨀똗㠑?⨀傗ꐂ㠑?⨀႘ꐂ餂돐リ�⨀ꢶ�⨀頿ﶖ⨀뤁뎀／ࠀ끶�⨀礁덐态Ȁ瀂莒莒Āā뤀댐�⨀螑㌄죞�⨀䀠�⨀ࡡ�⨀Ă頓煥獣愠獵扨敡瀀ｿｿｿｿ⡷�⨀⡷�⨀ꤠ돀遠�⨀堘�⨀႘�⨀脠돐�⨀ࢷ�⨀㢘�⨀夠뎐／ࠀჭ�⨀愁덀态Ȁヮ䞤烯墦Āā輀
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
