Abstract
Six Eco RI digested-DNA fragments, 17.6-kb, 8.3-kb, 7.3-kb, 10.5-kb, 5.0-kb, and 8.7-kb, were selected from genomic DNA of Pseudomonas gladioli pv. gladioli MAFF 302545 to obtain specific probes of this bacterium. The 8.3-kb fragment hybridized to 56 of the 61 strains of P. gladioli but did not hybridize to 46 strains of P. caryophylli, P. cepacia, P. glumae, P. plantarii and P. solanacearum with dot blot hybridization. This fragment hybridized to genomic DNA from the 56 strains of P. gladioli producing a single band of 8.3-kb with Southern blot hybridization. This implies that these P. gladioli strains contain an 8.3-kb DNA fragment of EcoRI termini, which is common to these strains. These results indicate that the 8.3-kb DNA fragment is useful as a probe for detection and rapid identification of P. gladioli.
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