A cleavage product of the adenovirus DNA binding protein is active in DNA replication in vitro

Abstract

The 72,000-dalton adenovirus DNA binding protein is coded by the adenovirus gene containing the H5ts125 mutation. The wild-type DNA binding protein can complement the H5ts125 temperature-sensitive defect in an in vitro DNA replication reaction. Chymotrypsin treatment of the 72,000-dalton DNA binding protein cleaved this protein into two fragments of 44,000 and 26,000 daltons. The 44,000-dalton polypeptide, derived from the wild-type adenovirus DNA binding protein, can replace the wild-type 72,000 dalton protein and complement the H5ts125 defect in the in vitro DNA replication system.