Abstract
Background: Endometrial cancer (EC) is one of the most common gynecological malignancies in women. Cholesterol metabolism has been confirmed to be closely related to tumor proliferation, invasion and metastasis. However, the correlation between cholesterol homeostasis-related genes and prognosis of EC remains unclear. Methods: EC patients from the Cancer Genome Atlas (TCGA) database were randomly divided into training cohort and test cohort. Transcriptome analysis, univariate survival analysis and LASSO Cox regression analysis were adopted to construct a cholesterol homeostasis-related gene signature from the training cohort. Subsequently, Kaplan-Meier (KM) plot, receiver operating characteristic (ROC) curve and principal component analysis (PCA) were utilized to verify the predictive performance of the gene signature in two cohorts. Additionally, enrichment analysis and immune infiltration analysis were performed on differentially expressed genes (DEGs) between two risk groups. Results: Seven cholesterol homeostasis-related genes were selected to establish a gene signature. KM plot, ROC curve and PCA in two cohorts demonstrated that the gene signature was an efficient independent prognostic indicator. The enrichment analysis and immune infiltration analysis indicated that the high-risk group generally had lower immune infiltrating cells and immune function. Conclusion: We constructed and validated a cholesterol homeostasis-related gene signature to predict the prognosis of EC, which correlated to immune infiltration and expected to help the diagnosis and precision treatment of EC.
Highlights
With about 382,000 new cases and 90,000 deaths worldwide in 2018, endometrial cancer (EC) is one of the most common gynecological malignancies in women, accompanied by the increasing incidence and decreasing age of onset (Bray et al, 2018; Siegel et al, 2019)
We analyzed the expression data of 74 genes related to cholesterol homeostasis in 552 EC samples and 23 normal samples in the Cancer Genome Atlas (TCGA) database to obtain 57 differentially expressed genes (DEGs) (p < 0.05, Supplementary Table S2)
Most of cholesterol homeostasis-related genes were differentially expressed between normal samples and EC samples
Summary
With about 382,000 new cases and 90,000 deaths worldwide in 2018, endometrial cancer (EC) is one of the most common gynecological malignancies in women, accompanied by the increasing incidence and decreasing age of onset (Bray et al, 2018; Siegel et al, 2019). The prognosis of EC patients at different stages are obviously different. With the rapid development of molecular biology and sequencing technology, some single-gene biomarkers have been unearthed to predict malignant tumors (Shang et al, 2018; Li et al, 2020a; Du et al, 2020). Endometrial cancer (EC) is one of the most common gynecological malignancies in women. The correlation between cholesterol homeostasis-related genes and prognosis of EC remains unclear
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