A Cholesterol and Actinide Dependent Shadow Biosphere of Archaea and Viroids in Neurodevelopmental Disorders

Abstract

AimEndogenous digoxin has been related to the pathogenesis of autism, cerebral palsy and trisomy 21. The possibility of endogenous digoxin synthesis by endosymbiotic bacteria with a mevalonate pathway and cholesterol catabolism was considered and explored in the study. MethodsCholesterol substrate was added to the plasma of the patients and the generation of cytochrome F420, free RNA, free DNA, polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, cytochrome C, hexokinase, ATP synthase, HMG CoA redutase, digoxin and bile acids were studied. The changes with the addition of antibiotics and rutile to the patient’s plasma were also studied.ResultsThe study showed rutile dependent increase in archaea and RNA viroids in the sera of these patients. The generation of cholesterol catabolites- polycyclic aromatic hydrocarbon, hydrogen peroxide, serotonin, pyruvate, ammonia, glutamate, digoxin and bile acids was increased in the presence of rutile suggesting a rutile dependent alternate biochemistry. The mevalonate pathway, ATP synthase and glycolysis in this archaea were also rutile dependent. The addition of antibiotics to the patient’s serum decreased all these activities suggesting their archaeal origin. ConclusionThe study demonstrates the existence of a shadow biosphere of rutile dependent archaea and viroids in these disease states. The archaeal cholesterol catabolism and viroidal RNA interference is crucial to the pathogenesis of these diseases. Key words: Autism; Cerebral palsy; trisomy 21; actinides; archaea; viroids; cholesterol oxidase; HMG CoA reductase