Abstract
Rubredoxin is one of the simplest iron–sulfur (Fe–S) proteins. It is found primarily in strict anaerobic bacteria and acts as a mediator of electron transfer participation in different biochemical reactions. The PutRUB gene encoding a chloroplast-localized rubredoxin family protein was screened from a yeast full-length cDNA library of Puccinellia tenuiflora under NaCl and NaHCO3 stress. We found that PutRUB expression was induced by abiotic stresses such as NaCl, NaHCO3, CuCl2 and H2O2. These findings suggested that PutRUB might be involved in plant responses to adversity. In order to study the function of this gene, we analyzed the phenotypic and physiological characteristics of PutRUB transgenic plants treated with NaCl and NaHCO3. The results showed that PutRUB overexpression inhibited H2O2 accumulation, and enhanced transgenic plant adaptability to NaCl and NaHCO3 stresses. This indicated PutRUB might be involved in maintaining normal electron transfer to reduce reactive oxygen species (ROS) accumulation.
Highlights
IntroductionPuccinellia tenuiflora, a graminaceous and alkali-tolerant halophyte species, is one such plant
P. tenuiflora has evolved various strategies to adapt to saline or alkaline stress, such as maintaining ion balance, osmotic homeostasis adjustment, and removal of reactive oxygen species (ROS) [10,11,12,13,14,15]
From the open reading frame (ORF), we deduced that PutRUB encoded a 225-amino acid protein with a predicted molecular mass of 27.38 kDa and a theoretical pI of 9.85
Summary
Puccinellia tenuiflora, a graminaceous and alkali-tolerant halophyte species, is one such plant It can complete its life cycle in highly alkaline soil (pH 10). P. tenuiflora has evolved various strategies to adapt to saline or alkaline stress, such as maintaining ion balance, osmotic homeostasis adjustment, and removal of reactive oxygen species (ROS) [10,11,12,13,14,15] It is considered a saline-alkali soil pioneer plant. We identified some candidate salt-responsive genes in P. tenuiflora using the Full-length cDNA Over-eXpressor gene (FOX)-hunting system This system is a very effective tool for plant functional gene research that does not require any knowledge of the genome of interest or genetic mapping. We hope these results will provide a theoretical basis for future exploration of the adaptation mechanism of P. tenuiflora
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