Abstract
In this work the authors have shown that stable gigaohm seals over micrometer-sized holes can be obtained in a time-frame of seconds by the electrophoretic self-positioning of charged lipid membranes. For the first time, single-channel events were recorded on silicon microstructures. Self-integrating channel-forming peptides, such as alamethicin are expected to have applications in screening assays based on single-channel recordings, including high-speed DNA sequencing and metabolite detection. In the future, this method could be extended by either functional reconstitution of ion channels into giant vesicles or their fusion (small proteoliposomes) into preformed bilayers. The possibility of combining this type of planar setup with high numerical optics may also enable the simultaneous observation of elec. and optical signals, which would help to elucidate the coupling between ligand-binding and channel events of ionotropic receptors. [on SciFinder (R)]
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
