Abstract
ObjectiveThis study was aimed to investigate the effects of JiaYanKangTai (JYKT) on regulating interleukin-17 (IL-17) signaling in rats with autoimmune thyroiditis.MethodsLewis rats were administrated with JYKT for eight weeks after a seven-week subcutaneous injection of thyroglobulin with adjuvant and feeding iodine water. Ultrasonography was performed and total volume of thyroid was calculated. The expressions of autoantibodies and hormones were detected. Morphological changes of thyroid were observed. Metabolomics profile and metabolic network analysis were conducted. IL-17 signaling was detected by polymerase chain reaction and immunohistochemistry separately.ResultsJYKT reduced the mean volumes of thyroid, decreased both levels of TPOAb and TGAb, and alleviated lymphocytic infiltration of the thyroid. Metabolic network analysis of metabolomics proved IL-17 signaling pathway as a critical pathway in JYKT administration for autoimmune thyroiditis. JYKT downregulated expressions of IL-17A, TRAF6, p-ERK1/2 and TNF-α.ConclusionJYKT alleviated inflammatory lesions of experimental autoimmune thyroiditis by regulating IL-17 signaling.
Highlights
Autoimmune thyroid diseases (AITDs) are a class of the most frequent organ-specific autoimmune diseases with a high genetic heritability of 55-75%, and the main causes of thyroid dysfunction clinically [1, 2]
Some randomized controlled trials reported that Traditional Chinese medicine (TCM) is an effective and alternative therapy for AITDs [7–9]
Thyroid volume can change over the course of autoimmune thyroiditis and it reflects changes in function of the thyroid
Summary
Lewis rats were administrated with JYKT for eight weeks after a seven-week subcutaneous injection of thyroglobulin with adjuvant and feeding iodine water. Ultrasonography was performed and total volume of thyroid was calculated. The expressions of autoantibodies and hormones were detected. Metabolomics profile and metabolic network analysis were conducted. IL-17 signaling was detected by polymerase chain reaction and immunohistochemistry separately
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