Abstract
The olfactory system serves a vital role in the evolution and survival of insects, being involved in behaviors such as host seeking, foraging, mating, and oviposition. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process. In this study, BtabCSP11, a CSP11 gene from the whitefly Bemisia tabaci, was cloned and characterized. The open reading frame of BtabCSP11 encodes 136 amino acids, with four highly conserved cysteine residues. The temporal and spatial expression profiles showed that BtabCSP11 was highly expressed in the abdomens of B. tabaci females. Dietary RNA interference (RNAi)-based functional analysis showed substantially reduced fecundity in parthenogenetically reproduced females, suggesting a potential role of BtabCSP11 in B. tabaci reproduction. These combined results expand the function of CSPs beyond chemosensation.
Highlights
The insect olfactory system is used extensively in a variety of contexts, such as during host seeking, foraging, mating, and oviposition behaviors
We explored the function of BtabCSP11 in B. tabaci
We carried out the following experiments: (1) we cloned and analyzed the structure of BtabCSP11, and constructed a phylogenetic tree to analyze its evolutionary relationship with other insect chemosensory proteins (CSPs); (2) we measured the temporospatial distribution of BtabCSP11 throughout different developmental stages and across different tissue types; and (3) we investigated the function of BtabCSP11 using dietary RNA interference (RNAi)
Summary
The insect olfactory system is used extensively in a variety of contexts, such as during host seeking, foraging, mating, and oviposition behaviors. Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the olfactory recognition process (Pelosi et al, 2014, 2018). Both OBPs and CSPs are globular water-soluble acidic proteins with low isoelectric points found at high concentrations surrounding the chemosensory neurons (Pelosi et al, 2006). CSPs are broadly expressed in non-chemosensory tissue, such as the subcuticular layer and wings (Marchese et al, 2000; Ban et al, 2003; Lu et al, 2007). The ubiquitous expression of CSPs suggests that, in addition to their known role in chemosensation, CSPs are involved in other physiological functions. Sexual behavior, mating, ovulation and oviposition are the main
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