A Chemosensing Ensemble for the Detection of Cysteine Based on the Inner Filter Effect Using a Rhodamine B Spirolactam

Abstract

A fluorescent chemosensing ensemble for the detection of cysteine is designed based on the fluorescence inner filter effect. The method employs the coordination of Cu(2+) ion with salicylaldehyde rhodamine B hydrazone (I), a colorless and non-fluorescent rhodamine B spirolactam derivative to form I-Cu(II), a pink color but weakly fluorescent complex. When rhodamine B was introduced to the I-Cu(II) complex solution, the fluorescence signal of rhodamine B is dramatically decreased because of the fluorescence inner filter effect (IFE). Upon adding cysteine to the above solution, it can complex preferentially to Cu(2+) compared to I, and the I-Cu(II) complex dissociates, which thus decreases the fluorescence IFE of the solution, and in turn leading to the fluorescence increase of the chemosensing system. Based on the above mechanism, a fluorescent chemosensing ensemble for cysteine is developed. The fluorescence increase is linearly with cysteine concentration up to 10.0 mu mol L(-1), with a detection limit of 1.4 x 10(-7) mol L(-1) (3sigma). The optimal conditions of the proposed method were studied and the selectivity of the proposed method was investigated in this paper.