A certain mutant blocked in trans-4-hydroxy-L-proline formation from L-proline during neoviridogrisein biosynthesis.

Abstract

A mutant blocked in l-proline hydroxylation was derived from a neoviridogriseins producing organism, Streptomyces griseoviridus P8648. This mutant, G-89, did not produce trans-4-hydroxy-l-proline which is the direct precursor of cis-4-hydroxy-D-proline in viridogrisein. Consequently, the organism selectively produced neoviridogrisein II in which the imino acid position is occupied by d-proline.