A century later: Tricolored Pied Flat Coladenia indrani uposathra Fruhstorfer, 1911 (Hesperiidae: Pyrginae) and Crenulate Oakblue Apporasa atkinsoni Hewitson, 1869 (Lycaenidae: Theclinae) reported from Manipur, India

Keibul Lamjao National Park (KLNP), Loktak lake Manipur, north-east India which is one of the 25 Ramsar sites of international importance and the biggest fresh water Lake in India. Macrophytes of the floating Phumdi mat environment of KLNP play an important role in the aquatic environment. An investigation was planned during the period of about three years from March, 2010 to December 2012, to study the diversity pattern of habitats of macrophytes available in six study sites of KLNP viz., 1.Kumbi, 2.Khordak, 3.Keibul, 4.Toya, 5. Nongmaikhong and 6. Sargam. All total 85 dominant plant species were recorded. Maximum plant species (49) was observed in Site-1 Kumbi (Altitude-780m) and minimum (27) in Site-3 Keibul (Altitude-772). The variation of plant species may be because of the slight variation of altitude. As the six study sites are distributed as floating aquatic environment in KLNP Loktak Lake, there is favourable place of the plants in higher altitude and altitude represents a complex gradient along which many environmental variables change concomitantly. In all the six study sites, common distribution of ten dominant plant macrophytes viz., Ageratum conizoides, Hedychium coronarium, Leersia hexandra, Oenanthe javanica, Phragmites karka, Polygonum sagittatum, Saccharum munja, Thelypteris interrupta and Zizania latifolia was noticed. Individual dominant plant was recorded in other sites also viz., Azola piñata in site-6; Xanthium atrumarium, Polygonum orientale, Dichrocephala latifolia and Cymbopogon citratus in site-2; Arundo plinii, Cuscuta reflexa, Gnaphalium luteo-album, Hydrilla verticillata, Marsilia minuta, Saccolepis interrupta, Selvenia cuculata and Utricularia spp. in site-1. Variation of IVI value of dominant plant species was observed in this study. Even though 85 dominant plants were selected for all the 6 sites, however, maximum plant richness expressed in IVI was recorded in case of Zizania latifolia (Site-5, IVI-87.5) and minimum IVI in case of Xanthium strumarium (Site-2, IVI-1.13). The diversity pattern and habitats of macrophytes in KLNP Phumdi environment might be due to water availability along the altitudinal gradient and other environmental factors suited in the study sites and expected to be an important factor affecting the survival and fecundity of plant population. We need to conserve the natural habitat of KLNP thereby maintaining the luxuriant growth of the seasonal and perennial, macrophytes, so as to maintain the natural flora and fauna of the park. These plants are the food of man and animal therefore, it needs a proper care and attention to protect them from over exploitation. Considering the diversity pattern of habitats of Macrophytes in KLNP, a floating mat Phumdi environment with high floral diversity and unique vegetation assemblage, it has been suggested that this track and adjoining sites should be declared as ecologically sensitive area not only the World Heritage Site.

Aquatic insect diversity of a protected area, Keibul Lamjao National Park in Manipur, North East India

An in-depth understanding of carbon dynamics and ecosystem productivity is essential for conservation and management of different ecosystems. Ecosystem dynamics and carbon budget are assessed by estimating net ecosystem production (NEP) across different global ecosystems. An ecological productivity assessment of forest and floating meadow ecosystems in Keibul Lamjao National Park (KLNP), Manipur, North East India, was conducted using the multi-criteria decision-making process namely, gray relational analysis (GRA). The analysis was performed on 24 selected criterions classified either as "higher-the-better" or "lower-the-better" based on their degree of influence on the carbon budget. Floating meadows exhibited a higher production of aboveground and belowground biomass and a higher total mortality and decay. Furthermore, the study found that floating meadows exhibited a higher soil organic carbon (SOC) and net soil organic matter (SOM) than the forest ecosystem. The forest ecosystem showed higher total respiration (RT), heterotrophic respiration (RH), and autotrophic respiration (RA) than floating meadows. Floating meadows exhibited a higher net primary productivity (NPP) of 616.49 ± 33.87 gCm-2year-1 than the forest ecosystem, which has a NPP of 566.64 ± 65.26 gCm-2year-1. Similarly, floating meadows have higher NEP (495.25 ± 36.46 gCm-2year-1) than forest ecosystems (418.39 ± 65.76 gCm-2year-1). These characteristics have a significant influence on the carbon budget in floating meadows as compared to forest ecosystems, as shown by larger values of gray relational coefficient (GRC) in GRA. The floating meadows ecosystem (0.82) obtained 54.72% gain in gray relational grades (GRG) value with the forest ecosystem (0.53). This study might help in improving KLNP and other adjutant areas for conservation and management policies from the vital information given on the importance of wetlands in carbon dynamics and ecosystem productivity.

The field survey carried out to document the baseline data of butterfly fauna Dhansiri Reserve Forest of Karbi Anglong District, Assam recorded 106 species belonging to six families. Graphium sarpedon, Appias albina, Spindasis lohita, Charaxes marmax, Athyma ranga, Tanaecia lepidae, Hypolycaena othona and Euploea mulciber are legally protected species under the Indian Wild Life (Protection) Amendment Act, 2022. Eurema andersonii, Appias albina, Appias galba, Charaxes marmax and Athyma ranga are some of the rare species recorded during the study. Record of rare and endemic species from this Reserve Forest, and presence of species legally protected under the Indian Wildlife (Protection) Amendment Act, marks its importance as an area for butterfly conservation.

Loktak Lake is the largest fresh water Lake in the North-eastern India. Keibul Lamjao National Park (KLNP) is a floating-mat (Phumdi) park inside the Lake. Study deals with the inter-related approaches: micobiological study, free-listing, participant observation and preference ranking of the six study sites of KLNP. Generally, plant collection per day was about 600 kg of Hedychium coronarium J. Koenig which were extracted from the park and found to be the most preferable species; while Persicaria sagittata (L.) H. Gross, as the least among the selected plants. Microbiological analysis of water for the six study sites recorded high pollution as evidenced by high values of Standard Plate Count (SPC) for bacteria ranging from 73,500-96,500 and microbial analysis of MPN (Most Probable Number) of coliform bacteria varies between100 and 320/100 ml and faecal coliform 95 and 200/100 ml. Free CO2 concentrations varied at the surface from 2-60 mg/l and 6-70 mg/l at the bottom of the park. In the KLNP environment the higher CO2 and lower concentrations of dissolved oxygen (DO) might be due to the floating mat (Phumdi) nature, in which light and exchange of gases does not occur as it does in the natural water bodies.

Odonata larvae were collected from Keibul Lamjao National Park, Manipur, northeastern India during 2009-2011. The study recorded 15 species of Odonata larvae belonging to the anisopteran families Aeshnidae, Libellulidae and zygopteran families Calopterigidae, Lestidae and Coenagrionidae. The study revealed that there is a need for a detailed study of Odonata fauna of the national park as thorough knowledge of the important species of a conservation area is very essential for proper management.

A survey carried out for thrips (Thysanoptera) at the Keibul Lamjao National Park, Manipur and at Manipur University campus, within the Indo-Burma hotspot region of northeastern India revealed the occurrence of Anaphothrips incertus (Girault) , Mycterothrips auratus Wang, Bamboosiella hartwigi (Pitkin), Euphysothrips minozzii Bagnall, Mycterothrips ricini (Shumsheer) , Dolichothrips citripes (Bagnall), Xylaplothrips flavitibia Ananthakrishnan & Jagadish and X. inquilinus Priesner . The o ccurrence of the first three species in India and the remaining five species in northeastern India is reported for the first time through the present study. In addition, a new species, Tylothrips samirseni sp. nov. is described.

Assessing seasonal variation of diffusive nitrous oxide emission from freshwater wetland in Keibul Lamjao National Park, Manipur Northeast India

A wetlandscape is a landscape characterized by the presence of numerous interconnected wetlands (Bertassello et al. 2018). Loktak located in the Manipur River basin in India is a unique wetlandscape that includes numerous floodplain wetlands and associated channels. It hosts the Loktak wetland, the largest freshwater body in the northeastern India, also designated as Ramsar site and the Keibul Lamjao National Park, the only floating national park in the world. Like any other wetlands in the world, Loktak wetlandscape is also under great threat due to the changes in the hydrometeorological conditions associated with climate change as well as the human interventions in the wetlandscape and its catchment. Loktak hydro-electric project, the major anthropogenic intervention in the wetlandscape has a huge impact on the hydrology and ecology of Loktak wetland complex and to Pumlen wetland complex to a less significant level (Trisal and Manihar 2002). This work investigates the multi- and cross-scale degradation of wetlands in the Loktak wetlandscape in a nested-framework by studying hydrogeomorphic dynamics at catchment scale, wetlandscape scale, and wetland complex scale. The catchment scale encompasses surrounding uplands and hillslopes. At the wetlandscape scale, which includes interconnected wetlands and associated channels, individual wetland boundaries are demarcated using historical Corona image and recent Sentinel-2 image. By comparing historic and recent boundaries, we found that six natural wetlands of area less than 1.5km2 have completely converted into other land use types and three small wetlands with area less than 1km2 get merged with bigger ones due to barrage-induced prolonged inundation. The wetland complex scale is a cluster of hydrologically interconnected wetlands of same or different types. Loktak wetland complex has not shown any prominent change in its extent, whereas Ikop and Pumlen wetland complexes have shrunken remarkably over time. The degradation of wetlandscape is evident from other factors such as proliferation and thinning of phumdis (floating biomass) and destruction of vegetation in the catchment. The cross-scale investigation suggests the influence of both natural and anthropogenic controls on the degradation of Loktak wetlandscape. The findings of this study and the protocols developed here will help to better understand the stressors of Loktak wetlandscape and elsewhere and could be instrumental in developing a conservation and management plan. Multi-scale management of wetlandscape include the catchment-scale measures such as afforestation, protection of hills and reducing the frequency of shifting (jhum) cultivation in hilly areas, wetlandscape scale measures such as control of waste dumping, control of channel modification for builtup and then wetland scale measures such as removal of invasive species from open water, and control of athaphum farming (method of farming using phumdis). Considering that a huge proportion of population in the valley depend on these wetlands for their livelihood, accounting their needs and making them a part of any effort for the management of this ecosystem has to be one of the primary goal.

A three year study (from December 2011 to December 2014 and in June 2015) on butterflies covering four major forest sub-types as classified by H.G. Champion & S.K. Seth in 1968 in ‘Forest Types of India’, which occupy 60% of the forest area lying below 2,500m across Arunachal Pradesh State in the eastern Himalaya of India, revealed 415 taxa belonging to six families (Hesperiidae: 74 species of 42 genera; Papilionidae: 37 species of 10 genera; Pieridae: 36 species of 15 genera; Lycaenidae: 85 species of 49 genera; Riodinidae: 7 species of 3 genera & Nymphalidae: 176 species of 71 genera, respectively). These included many endemic and rare species typical of these forest sub-types, i.e., (i) 2B/1S1 Sub-Himalayan Light Alluvial Semi-Evergreen Forest (32 species), (ii) 2B/ C1(a) Assam Alluvial Plains Semi-Evergreen Forests (5 species), (iii) 2B/2S2 Eastern Alluvial Secondary Semi-Evergreen Forests- (15 species) and (iv) 3/1S2 (b) Terminalia-Duabanga (3 species), respectively. The relative number of species and individuals sampled were the highest at altitudes below 500m, and gradually declined as the altitude increased to 2,000m, and above 2,500m species richness declined sharply. The number of species and their relative abundance were the highest during July–August (Monsoon-first peak) and then again in November-December (Autumn-second peak), while the numbers were lowest during winter. These findings suggest that these four forest types are important both for the purpose of ecotourism as well as conservation of endemic and rare taxa found in the eastern Himalaya and northeastern India at altitudes below 2,000m. A complete list of all the taxa sampled is given along with relative abundance status during sampling, Indian Wildlife (Protection) Act, 1972 status, and distribution in different forest types in the state. Ten potential butterfly ecotourism zones are suggested for the state. Planning land-use for biodiversity conservation based on butterfly-forest type associations, by taking forest sub-types as units of conservation, is suggested as an option for the eastern Himalaya.

The hog deer (Axis porcinus) is threatened by habitat alteration, fragmentation, and poaching, which have led to a drastic decline of its wild population. Two subspecies of A. porcinus have been described from its distribution range. A. p. porcinus is reported to occur from Pakistan along the Himalayan foothills through Nepal, India and Myanmar, and A. p. annamiticus is found in Thailand, Indo-China, Laos, Cambodia, and Vietnam. However, the current distribution range of A. p. annamiticus is still unclear. We used the partial control region (CR) of mitochondrial DNA (mtDNA) and seven microsatellite loci to investigate the intra-species structure, differentiation, and demographic history of hog deer populations from three landscapes, the Terai Arc, Northeast, and Indo-Burma (Keibul Lamjao National Park (KLNP), Manipur, India) landscapes. We also carried out divergence time estimation using the complete mitogenome. The level of variation was ~4%, and the time of divergence of the KLNP population and the other Indian populations was about 0.22 Mya, i.e., during the last glaciation periods of the Late Pleistocene/Early Holocene. The KLNP haplotypes of the control region were shared with the Southeast Asian subspecies, A. p. annamiticus. The results of the investigations of the microsatellite loci supported the mtDNA results unambiguously. Two genetically distinct lineages are found in India: one is found from the Terai Arc to Assam (A. p. porcinus) and the other in Manipur (A. p. annamiticus). The genetic diversity in KLNP was low and exhibited a higher degree of genetic differentiation compared with major Indian populations. The Bayesian skyline plots indicated that after a long phase of historic demographic stability, the populations of both the lineages of hog deer suffered pronounced declines during the period from ~800 years BP to 5000 years BP. In summary, our finding provided evidence that the KLNP population is probably a prime, isolated and sustaining stock of A. p. annamiticus and should be managed as evolutionarily significant units (ESUs).

Seasonal variations in the water quality of a tropical wetland dominated by floating meadows and its implication for conservation of Ramsar wetlands

The Sangai deer (Rucervus eldii eldii) is an endangered species which is found only in KeibulLamjao National Park, Manipur (India) with an area of 40 km2. Selection of observation point and direct observation method were utilized to record certain behavioral pattern shown by the Sangai deer. Determination of food resources were carried out to identified feed species during the observation hour. The finding of the present study shows that the behavioral activities were notice more in the morning (dawn) than evening (dusk). The most favorable food by Sangai deer were Zizania latifolia (Kambong), Pragmitis karka (Tou), Saccharum munja (Khoimom), Carex cruciata (Humdang), Learsia hexandra (Hup), Alpinanigra (Pullei) and Hydechiumcoronarium (Loklei). Amongst the favorable food species, the most preferable food by the Sangai deer is Zizania latifolia while some of the individuals were seen consuming ashes (Tanggoi) during the observation. Intrusion of domestic animals within the park area may lead to severe calamities which then lead to the spread of many harmful diseases to Sangai deer and its inhabitants. Shyness of animal and sensitive behaviors of Sangai deer was observed. Questionnaire surveys were carried out among 40 respondents. The result suggested that the status of feeding must be taken care so that there should not be any alteration or changes in their behavior due to intrusion, local vegetables collector and disturbances.

The Garo Hills of Meghalaya, northeastern India, form the westernmost boundary of the globally recognized Indo-Myanmar Biodiversity Hotspot. The butterfly fauna of the Garo Hills is expected to be diverse, but it has not been properly sampled before. We surveyed butterflies in Balpakram National Park, Baghmara Reserve Forest and Siju Wildlife Sanctuary in southern Garo Hills, and Nokrek National Park in Western Garo Hills during four visits amounting to 49 days spread over two seasons, pre-monsoon (April-May) and post-monsoon (November-December), between 2008 and 2010. Here we report 298 butterfly species for the Garo Hills, eight of which are legally protected under Schedule I and 33 under Schedule II of the Indian Wildlife (Protection) Act, 1972. Our species accumulation curve suggests that many species remain to be discovered in the Garo Hills, and we expect the total species richness to be closer to 600-650 species. Little quantitative information exists on populations and seasonal occurrence of butterflies in India. Therefore, we recorded the number of individuals of each species in one or three hour counts during our surveys, and here we report season-wise relative abundances of 298 species from 3,736 individuals. We also report significant range extensions of two Schedule I species: Elymnias peali and Prothoe franck regalis, from the Garo Hills. These findings underscore the significance of the Garo Hills for butterfly conservation in India, and our work forms a baseline for future quantitative work on the diversity and conservation of butterflies in this biodiversity hotspot.

Since 28th May 2001, Whale shark Rhincodon typus Smith, 1828 have received the highest protected status for an animal in India through the Indian Wildlife (Protection) Act, 1972 Schedule-1. However, landings have still been recorded off the Indian coast since 2001, mostly as incidental bycatch in commercial fishing operations, and other sightings have also been reported. In the 1990’s, a targeted whale shark fishery existed off the Gujarat coast following increased demand for the flesh in some other Asian countries. Since the ban, landings of whale sharks have decreased substantially with only 79 recorded between 2001 and 2011. Landings were recorded in each year and in each month of the year with the highest landings in January and February. Between 2001 and 2011, the smallest specimen reported from Indian waters was a 94 cm TL individual and the largest was a 13.7 m TL individual, with most individuals recorded in the 4–6 m TL size class. Small juveniles of less than 3 m TL are rarely recorded in the literature and appear to be rarely observed globally. Between 2006 and 2011, seven juveniles of less than 3 m TL were recorded from two landing sites. Despite the continued landing of whale sharks along the Indian coasts since 2001, the protection of this species appears to have substantially reduced the catches with only incidental landings and strandings now evident. The protection status of whale sharks in India is generally well understood by fishers, but still there is need for further education regarding the current national legislation and vulnerability of the species.