A Central Repository for Published Plasmids

Abstract

To study the function of a segment of DNA, researchers typically insert it into a DNA-based vector called a plasmid. Plasmids can then be used in a variety of experimental systems. Circulation of plasmids among the academic community is essential for scientific progress but is hindered because of inefficiencies in the current distribution process. When a plasmid appears in an academic publication, it is considered to be public domain and should be made available to the scientific community upon request. Each laboratory presently stores its own published plasmids and is responsible for answering requests in a timely fashion. However, turnover of students and post-doctoral fellows and busy research schedules contribute to variable plasmid quality and delays in shipment. Addgene, a nonprofit organization, is creating a central plasmid repository where scientists can search for and request plasmids online. A plasmid repository will provide standardized quality control and reliable deliveries and will serve as an archive to prevent plasmid and information loss over time. Addgene accepts plasmids in either DNA or bacterial form and stores them as bacterial glycerol stocks both on-site and at a backup facility off-site. Vector maps and information regarding plasmid construction will be submitted by the originating laboratory and will be available on Addgene's Web site. Addgene will distribute plasmids through direct online ordering. Addgene's Web site will also contain a searchable database of its stored plasmids, making it convenient to locate all available plasmids containing different versions of a particular gene, such as tagged versions, dominant active or negative mutants, or those expressed in unique vectors. Addgene will not only collect plasmids from newly published papers, but will also retroactively collect plasmids from those investigators who would like to submit frequently requested plasmids. By doing so, Addgene will relieve investigators of the time and effort needed to answer requests, as well as provide a reliable archival service for plasmids and maps. All scientists who submit plasmids to Addgene will have access to the list of researchers who have requested their reagents. Addgene is currently working with high-impact journals to collect plasmids at the time of publication. Addgene invites the scientific community to participate in this endeavor. Investigators and journals that would like to partner with Addgene to build this resource are welcome to submit plasmids or learn more about the initiative by visiting .