A cellulose/β-cyclodextrin nanofiber patch as a wearable epidermal glucose sensor

Kyu Oh Kim,Geon Jin Kim,Ji Hye Kim

doi:10.1039/c9ra03887f

Kyu Oh Kim, Geon Jin Kim + Show 1 more

Open Access

https://doi.org/10.1039/c9ra03887f

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Journal: RSC advances	Publication Date: Jan 1, 2019
Citations: 34	License type: CC BY 3.0

Affiliation: Dankook University, Catholic University of Korea

Abstract

In this study, we aimed to develop a cellulose/β-cyclodextrin (β-CD) electrospun immobilized GOx enzyme patch with reverse iontophoresis for noninvasive monitoring of interstitial fluid (ISF) glucose levels (0.1–0.6 mM dm−3). In vitro analysis, performed using a sensor attached to flexible substrates, revealed that the high diffusion coefficient (9.0 × 10−5 cm2 s−1), the linear correlation coefficient (R2 = 0.998), the detection limit (9.35 × 10−5 M), and the linear range sensitivity (0–1 mM) of the sensor (5.08 μA mM−1) remained unaffected by the presence of interfering substances (e.g., fructose, sucrose, uric acid, and acetaminophen) at physiological levels. The present results indicate that the new epidermal sensing strategy using nanofibers for continuous glucose monitoring has potential to be applied in diagnosis of diabetes.

Highlights

Wearable and mobile technologies have received increasing interest in recent years, which has led to increased efforts toward the development of noninvasive glucose monitoring platforms
We aimed to develop a cellulose/b-cyclodextrin (b-CD) electrospun immobilized glucose oxidase (GOx) enzyme patch with reverse iontophoresis for noninvasive monitoring of interstitial fluid (ISF) glucose levels (0.1– 0.6 mM dmÀ3)
For stabilizing enzyme and accurate detection of redox active of cellulose/b-CD nano ber, a hydrophobic carbon chain of GOx were inserted into b-CD cavity via host–guest interactions

Summary

Introduction

Wearable and mobile technologies have received increasing interest in recent years, which has led to increased efforts toward the development of noninvasive glucose monitoring platforms. Continuous glucose monitoring overcomes the limitations of nger-stick blood sampling, thereby facilitating optimal therapeutic interventions. Reverse iontophoresis was developed by Guy's group for noninvasive glucose monitoring using biosensors.[1,2,3,4] When a small current is applied across the skin, the substance of interest (such as glucose) can be withdrawn from within or beneath the skin to the epidermal surface for detection. A biosensor platform with a low detection limit, high sensitivity, and high stability for longterm monitoring is warranted to measure glucose levels extracted by reverse iontophoresis.[6]

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