Abstract

Broth cultures of Mycoplasma synoviae WVU 1853 and M. gallisepticum S6 and X95 were fractionated by centrifugation into a deposit of cells, and a substantially cell-free supernatant. Haemagglutinating activity was found in the supernatant of all three strains. This was particularly marked with M. synoviae and, on a volumetric basis, the supernatant had much more activity than the cells. M. synoviae culture supernatant antigen had sensitivity and specificity similar to conventional whole culture antigen in haemagglutination inhibition tests against known M. synoviae, M. gallisepticum and negative sera. By use of supernatant for the HI test, and harvested cells for preparation of rapid slide agglutination antigen, two products can be obtained from one batch of culture.

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