Abstract
The Ml cell line (Clone M1/436.7) established from a myeloid leukemia of an SL strain mouse by Y. Ichikawa (1) is known to be inducible to differentiate into mature macrophages by exposure to various inducers, such as lipopolysaccharide (LPS) (2). Employing these clonal cells, we tested various macrophage activating agents for their possible differentiation inducing ability. As summarized in Figure 1, crude lymphokines, obtained by stimulating normal mouse spleen cells with concanavalin A-agarose, induced Ml cell differentiation, while neither purified interferon (IFN; 107.3 units/mg; given by Haruo Kono, NIH, Tokyo) nor synthetic muramyl dipeptide (MDP) did so (3,4). Instead, IFN enhanced Ml cell differentiation triggered by LPS or lymphokines, and MDP acted on matured M1 cells to produce factor(s) which could trigger M1 cell differentiation (4).
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