Abstract
A cell line from a human salivary-gland carcinoma.
Highlights
IN THE COURSE of studying the morphological and biological characteristics of human salivary-gland tumour cells (Takeuchi et al, 1975, 1976), we have established a long-term cell line from a squamous-cell carcinoma of the parotid
During the first 2 months after incubation, a mass of polygonal epithelial cells was visible surrounded by fibroblastic cells, the scattered epithelial cells growing out and resembling paving stones
The first subculture was taken on the 180th day with EDTA-trypsin
Summary
IN THE COURSE of studying the morphological and biological characteristics of human salivary-gland tumour cells (Takeuchi et al, 1975, 1976), we have established a long-term cell line from a squamous-cell carcinoma of the parotid. Small segments of tumour tissue were taken from one of the largest cervical lymph nodes, which was confirmed histologically as having the metastatic foci of squamouscell carcinoma with a large amount of fibrous connective-tissue stroma (Fig. 1). The culture bottles were incubated at 37°C and fed twice a week by replacement of the medium. The cells were separated from the glass surface by EDTA-trypsin solution, collected by centrifugation, and resuspended in fresh medium for the passage.
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