Abstract
Studies on the adult Drosophila midgut have led many insights on our understanding of cell type diversity, stem cell regeneration, tissue homeostasis and cell fate decision. Advances in scRNA-seq provide opportunities to identify new cell types and molecular features. We used inDrop to characterize the transcriptome of midgut epithelial cells and identified 12 distinct clusters representing intestinal stem cells (ISCs), enteroblasts (EBs), enteroendocrine cells (EEs), and enterocytes (ECs). This unbiased approach recovered 90% of the known ISCs/EBs markers highlighting the high quality of the dataset and led to new insights on ISCs cell biology, the roles of new transcription factors, and EEs hormonal expression diversity. To facilitate, mining of the richness of the data set, we provide a web-based resource for visualization of gene expression in single cells. Altogether, our study provides a comprehensive resource for addressing novel functions of genes in the midgut epithelium.
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