Abstract

The role of ethylene in keeping the apical hook of many etiolated dicotyledonous seedlings tightly closed is well established (Abeles, 1973). The hook results from an asymmetry in growth between the upper and the lower sides of the stem. There is evidence that this differential growth may be the result of asymmetric distribution of ethylene biosynthesis (Schierle and Schwark, 1988). To study the distribution and regulation of the ethylene biosynthetic enzymes in apical hooks of pea (Pisum sativum L. cv Alaska), it is necessary to isolate clones encoding ACC synthase and the ethyleneforming enzyme, ACC oxidase.

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