Abstract
Pulse-labeling of an Escherichia coli strain harboring the resistance transfer factor R6K results in a transient increase in labeled catenated R6K DNA molecules. After a chase the level of labeled catenated DNA molecules is greatly reduced concomitant with a marked increase in labeling of the supercoiled DNA form of R6K. The data presented support a role for the catenated DNA molecule as an intermediate in the replication of the plasmid R6K.
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Schedule a callMore From: Biochemical and biophysical research communications
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