Abstract
The discharge from wastewater treatment plants (WWTPs) into the receiving river is of concern for the health of the ecosystem. In this study, the microbial diversity impact of Fuxin Meng (FM) WWTP was completed with three pairs of primers. At the time of impact assessment, biochemical tests were carried out as well to investigate the water quality downstream and upstream FMWWTP. The general results showed this plant increased downstream biochemical oxygen demand (BOD) and chemical oxygen demand (COD) by 4.3- and 8.1-fold respectively, in comparison with the upstream. The microbial diversity impact from the plant was also investigated with three pairs of universal primers targeting rRNA genes and the internal transcribed spacer (ITS) by high-throughput sequencing. The downstream diversity of the bacterial and fungal communities was generally higher than upstream, with the exception of the eukaryotic community. Both biochemical and microbial assessments suggested that FMWWTP deteriorate Xi River water quality, potentially acting as a pollutive source. Of the receiving water. Accordingly, it was necessary to improve the operation of WWTP to live up to the environmental quality standards.
Highlights
Discharge from WWTPs often disturbs the natural microbial communities of the receiving waterbodies
It was necessary to improve the operation of WWTP to live up to the environmental quality standards
The total nitrogen (TN), BOD5, and chemical oxygen demand (COD) at both sites indicated that the plant did not adequately treat wastewater
Summary
Discharge from WWTPs often disturbs the natural microbial communities of the receiving waterbodies. The impact caused by WWTP on the microbial communities of the river is of warm concern for environmental management. Studying the microbial diversity of river-water may help to improve the wastewater treatment facilities or to identify any healththreatening factors. It is necessary to promptly investigate the microbial diversity of the Xi River water subjected to the FMWWTP discharge (Yotova et al, 2019). The prokaryotic and eukaryotic microbial community diversities of the samples were surveyed by sequencing the 16S and 18S rRNA genes, respectively. This sequencing-based method of conserved genes is independent of culturing or microscopy.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
