Abstract
Carnivores are an interesting model for studies of embryonic amino acid metabolism and ammonium (NH4+) toxicity given the high-protein content of their diets. Our objectives were to examine concentration- and stage-specific effects of essential amino acids (EAA; 0×, 0.125×, 0.25×, 0.5×, or 1.0× the concentrations in Minimum Essential Medium) and NH4+ (0, 300, or 600 μM) on the development and metabolism of feline embryos. The presence of EAA, regardless of concentration, during days 3-7 of culture increased (P<0.01) the proportion of embryos that initiated hatching (>14.3%) and the total number of cells per blastocyst (>148.3 cells) compared to embryos cultured without EAA (0.0% and 113.2±3.7 cells, respectively). The presence of EAA during days 1-3 (0.25×) and 3-7 (1.0×) of culture increased (P<0.01) the proportions of embryos that formed blastocysts (82.9±4.2%) and initiated hatching (32.9±5.2%), and the number of cells per blastocyst (247.9±12.1 cells), compared to control embryos (60.0±5.3%, 0.0%, 123.2±8.1 cells, respectively). The presence of NH4+ in the medium did not affect (P>0.05) development of feline embryos. The addition of EAA or NH4+ during culture did not affect (P>0.05) the production of Gln by feline embryos, but decreased (P<0.05) production of Ala and increased (P<0.05) production of urea. Additional work is needed to determine if our observations are unique to feline embryos or reflect an adaptation to a high-protein diet that is conserved in other carnivores.
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