A caprine experimental model for studies on inflammation.

Abstract

A new experimental model for in vivo studies on local inflammation in the goat is presented. The teat and udder cisterns were separated by a surgical procedure, resulting in the teat cistern being an isolated pouch which is easily accessible through the teat canal and suitable for experimental studies. The surgery was consistently successful in closing the passage and no post-surgical complications were observed. The model was applied to a study of the inflammatory response induced by infusion of Salmonella endotoxin. A marked response was observed as measured by the accumulation of leukocytes, serum albumin and N-acetyl-beta-D-glucosaminidase (NAGase) in the test cistern. An initial increase in serum albumin, indicating an increase in the epithelial permeability, was observed from 1.5 h after endotoxin infusion. Approximately 0.5 h later, the cell count started to increase, reaching its peak level 3 h after infusion. The NAGase concentration was closely correlated with the cell count. The model provides new possibilities for in vivo studies on local inflammation and fulfils many of the requirements of an inflammatory model; for example, it allows non-traumatic repeated samplings from the same animal. The goat is a suitable experimental animal for many studies and, as each goat has two teats, intra-goat comparisons can be performed.