Abstract
Capillary electrophoresis (CE) has been used to develop a method that allows analysis for all the main components of Eminase. This technique has also resolved the two glycosylated forms of lys-plasminogen. Quantitative CE analysis of concentration levels of this enzyme complex have been directly compared to those obtained from a bio-assay. A linear relation between the two methods has been demonstrated. Moreover, the CE method is highly reproducible, combines precision with speed, and allows quantitative analysis of the other protein components (streptokinase and human serum albumin) in Eminase.
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