A cancer cell and lysosome dual-targeted photosensitizer for fluorescence imaging and photodynamic therapy

Abstract

A novel cancer cell and lysosome dual-targeted photosensitizer (PS) termed by BMP was developed for fluorescence imaging and photodynamic therapy (PDT). BMP consists of a porphyrin(Zn) core with appropriate singlet-triplet state distribution enables it to function as an imaging agent as well as a PS, a biotin moiety that can target cancer cells, and a morpholine group specific to lysosomes. Intracellular experiments demonstrated that the dual-targeted photosensitizer BMP could preferably accumulate in cancer cells and subsequently locate within lysosome organelles. Upon irradiation, BMP could generate red fluorescence emission (Φem = 6.3%) and singlet oxygen (1O2, ΦΔ = 55%) for the dual functionality of cell imaging and PDT, respectively. Notably, BMP exhibited much higher photocytotoxicity toward cancer cells (A549 cells) compared to normal cells (BEAS-2B cells). Importantly, 1O2 produced by BMP induced oxidative damage of lysosomes, and subsequently triggered cell death of A549 cells, as evidenced by the loss of morphological integrity and cell rupture.