Abstract
Complex, mobile spherical structures continuous with the vacuolar limiting membrane were identified in young cotyledons of Arabidopsis by expression of a vacuolar membrane marker(r-TIP-GFP)and by electron microscopy, and were named“bulbs”. These structures are cytoplasm wrapped by two layers of intricately folded membrane; both layers are continuous with the vacuolar limiting membrane. When visualized using r-TIP-GFP, the fluorescence intensity of bulbs is about3-fold higher than that of the vacuolar limiting membrane. However, bulbs were negative for GFP-AtRab75, another vacuolar marker. These results suggest that bulbs form a subregion in the continuous vacuolar membrane that concentrate some proteins and segregate others.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
