Abstract
Cell proliferation was studied in pre-odontoblasts, and in cells of the dental papilla and lingual dental follicle using bromodeoxyuridine immunocytochemistry and morphometry in Bouin's perfused and paraffin-embedded, undemineralized maxillary incisors. Cells in DNA synthesis, as shown by this technique, or in mitosis, were counted. Significantly fewer labelled nuclei, unlabelled nuclei and total nuclei were found in the tissues of growth hormone-deficient dwarf rats than in normal tissues. However, in dwarf rats treated for 6 days with bovine growth hormone, their numbers were equivalent to, or in some instances greater than those in normal tissues. The bromodeoxyuridine labelling index, the ratio of positive to negative nuclei and the mitotic index of pre-odontoblasts in dwarf rats were consistently lower than in normal rats, and were reversible by growth hormone. Growth hormone thus plays a part in odontogenic mesenchymal proliferation.
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