A broad-host range coliphage against a clinically isolated E. coli O157: isolation and characterization

Abdollah Ghasemian ,Maryam Bavand ,Zahra Moradpour

doi:10.7324/japs.2017.70320

Abstract

Isolation of colicinogenic E. coli, optimization of colicin production and transformation of Col plasmidArumugam Suresh, Manikkam Radhakrishnan1, Gopal Suresh, Nainangu Prasannabalaji, Sivaraj Anbarasu, Jerrine Joseph

Highlights

Despite the strict controls to prevent food born diseases; enterohaemorrhagic Escherichia coli (E. coli) E. coli (EHEC) serogroup O157 has emerged as a worldwide threat to public health following its first identification in an outbreak occurred by year 1982, when the illness was associated with the consumption of undercooked ground beef (Aslantas et al, 2006; Dontorou et al, 2003)
The results indicated that isolated phage has a lytic effect on 3 of 13 representative bacterial species as well as E. coli O157. gBSN-MGB13 produced plaques on Bacillus subtilis (B. subtilis), Proteus vulgaris (P. vulgaris) and Pseudomonas aeroginosa (P. aeroginosa)
Some studies have been carried out to assess the abilities of the phage to fight E. coli O157 (Bach et al, 2003; Kudva et al, 1999; Tanji et al, 2005)

Summary

Introduction

Despite the strict controls to prevent food born diseases; enterohaemorrhagic Escherichia coli (E. coli) E. coli (EHEC) serogroup O157 has emerged as a worldwide threat to public health following its first identification in an outbreak occurred by year 1982, when the illness was associated with the consumption of undercooked ground beef (Aslantas et al, 2006; Dontorou et al, 2003). According to the U.S center for disease control and prevention (CDC), E. coli O157 is still an important cause of human illness in the United States and CDC reports multistate outbreaks of E. coli O157 regularly in which one of the last cases was in 2014 (Centers for Disease Control and Prevention, 2014). This food-borne pathogen is the reason of Ghasemian et al / Journal of Applied Pharmaceutical Science 7 (03); 2017: 123-128. The isolated phage is broad-host range and can kill Bacillus subtilis, Proteus vulgaris and Pseudomonas aeruginosa, as well This lytic phage can potentially be used in controlling these four pathogens

Objectives

Methods

Results

Conclusion