Abstract
As the first and largest user of next-generation Illumina HiSeq 2000 (HiSeq) sequencers in the world, Beijing Genomics Institute (BGI) has extensive experience utilizing HiSeq, covering almost all sequencing areas, including human, animal, plant and microbe, and almost all sequencing types, including de novo sequencing, resequencing, metagenome sequencing, RNA-Seq, Digital Gene Express. BGI has managed to convert the full sequencing power from 20 Genome Analyzer II (GA) to, currently, 100 HiSeq 2000 sequencers, with the useful supplementation of 15 SOLiD™ 4 systems from Applied Biosystems. A brief evaluation and comparison of the two Illumina sequencing systems is provided in this manuscript, including library construction, quality control, multiplexing and automation, as well as troubleshooting guidelines and a few demo cases utilizing HiSeq 2000 at BGI.
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