A Blockade of IL‐6 Released from Human Breast Carcinoma MDA‐MB‐231 in Presence of NK‐92 Inhibits an Invasion of Breast Carcinoma

Abstract

A variety of cytokines are existed in tumor microenvironment, which might affect tumorigenesis and metastasis. Recent studies have demonstrated that interleukin(IL)‐6, a pro‐inflammatory cytokine, is significantly present in breast tumor microenvironment. It is suggested to have a critical role in an epithelial to mesenchymal transmission(EMT) process in breast cancer cells. Natural killer (NK) cells are a large innate lymphocyte that has a cytotoxic effect to cancer cells or virus‐infected cells. However, in breast tumor microenvironment, NK cells are shown to have pro‐tumor activity in presence of various inflammatory cytokines and growth factors. We found that IL‐6 is highly produced in coculture of invasive breast cancer cell MDA‐MB‐231 and natural killer(NK)‐92 cell, but is little produced in coculture of non‐invasive breast cancer cell MCF‐7 and NK cells. Subsequently, we found that the expression of p‐stat3, p‐erk, akt and pi3k proteins is statistically up‐regulated in a coculture of MDA‐MB‐231 and NK‐92 compared to in coculture with MCF‐7. A blockade of IL‐6 by anti‐IL‐6 (1ng/ml) or shRNA in invasive breast cancer cell MDA‐MB‐231 reduced a migratory and invasive capability as well as down‐regulated an expression of p‐stat3 protein. Further we examined the inhibitory effect of apigenin on invasion or migration of invasive breast cancer cell MDA‐MB‐231. We found that a blockade of IL‐6 by apigenin in MDA‐MB‐231 mediates the inhibitory effect on migration and invasion of MDA‐MB‐231.Support or Funding Informationthis research was supported by basic science research program through the national research foundation of korea(nrf) funded by the ministry of education(2016R1A6A1A03007648)This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.