A blind deconvolution method in a LCSM system

Abstract

The Laser Confocal Scanning Microscopy (LCSM) system is one kind of modern microscopic instruments characterized by its depth discrimination capability. Nevertheless, the quality of confocal microscopy images suffers from two basic physical limitations. The image produced by LCSM can therefore benefit from post-processing by deconvolution methods designed to reduce blur or noise. In this paper we present an improved blind deconvolution algorithm in which the initial value of parameters of PSF is determined based on error analysis. The results show that this method can effectively restore the degraded image acquired from the LCSM system.