Abstract
ABSTRACTFeed additives that promote gastrointestinal health may complement coccidiosis vaccination programs in antibiotic-free broiler production systems. This study examined the effects of a commercial feed additive blend (FA) on intestinal histomorphology and inflammatory biomarkers in vaccinated Ross 708 cockerels (N = 2,160). The study was a randomized complete block design (12 blocks) with 3 dietary treatments: CON (negative control), AGP (positive control: 55 ppm of bacitracin methylene disalicylate), and FA (1.5 kg/MT in starter; 1.0 kg/MT in grower; and 0.5 kg/MT in finisher). Birds were reared on re-used litter and fed a 3-phase feeding program (starter, 0 to 14 D; grower, 15 to 28 D; finisher, 29 to 36 D). One master batch of basal feed for each feeding phase was prepared and final experimental diets were manufactured by mixing the basal feed with the respective test ingredient prior to pelleting. Growth measurements, including pen body weight and feed intakes, and fresh fecal samples were taken throughout the study. On day 20, samples of intestinal tissue were collected from a subset of birds (n = 72, 1 block) for histomorphology and mRNA expression of tight junction and inflammatory genes. In the duodenum, the ratio of villi length to crypt depth was significantly lower in FA (and AGP) fed birds than those consuming the CON diet. Relative mRNA expressions of iNOS, IFNƔ, and claudin-1 were upregulated in the jejunum of FA and AGP treatment groups compared to those in the CON group; the response in the FA was of lesser magnitude than AGP. Together, these results demonstrated that the FA treatment altered the microstructure of the duodenum and affected the expression of inflammatory genes in the jejunum. The timing of these changes coincided with peak oocyte shedding in feces and an observed reduction in feed efficiency in all dietary treatment groups.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
