Abstract
Hydrogen sulfide (H2S), a well-known signaling molecule, exerts significant regulatory effects on the cardiovascular and nervous systems. Therefore, monitoring the metabolism of H2S offers a potential mechanism to detect various diseases. In addition, biotin is significantly used as a targeting group to detect cancer cells exclusively. In this work, a biotin-guided benzoxadizole-based fluorescent probe, NP-biotin, was developed for H2S detection and evaluated in normal liver cell (LO2) and liver cancer cell (HepG2) lines. Results reveal that NP-biotin can detect cellular H2S with high sensitivity and selectivity. Moreover, NP-biotin has been confirmed to possess the ability to target cancer cells under the guidance of the biotin group.
Highlights
Like carbon monoxide (CO) and nitric oxide (NO), hydrogen sul de (H2S) is well known as a gaseous mediator
Many studies have suggested that endogenous H2S is mainly produced from cysteine by CBS or CSE enzymes, which are responsible for the synthesis of H2S in vivo.[10,11,12]
The stock solution of NP-biotin was diluted to work solution (10 mM) by PBS (10 mM, pH 1⁄4 7.4, 1% DMSO)
Summary
Like carbon monoxide (CO) and nitric oxide (NO), hydrogen sul de (H2S) is well known as a gaseous mediator. A biotin-guided piperazinyl-NBD-based uorescent probe NP-biotin for H2S is reported. 3.69 nM, which is much lower than the physiological concentration of H2S (10–100 mM).[36,37,38] NP-biotin exhibited great sensitivity for Na2S, which indicates its good potential in living cell imaging.
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