Abstract
An attempt has been made to enhance colchicine biosynthesis in Iphigenia indica L grown in vitro by exogenous supply of precursors and elicitors during culture. Addition of tyrosine at 30 mg l−1 enhanced colchicine accumulation up to 6.1 mg g−1 dry wt (control 0.99 mg g−1 dry wt), while 25 μM NiSO4 stimulated maximum colchicine accumulation (10.25 fold of control value). The colchicine accumulation was recorded as 16.25 mg g−1 dry wt when NiSO4 was added with tyrosine.
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