Abstract
We developed a biosensor that is capable for simultaneous surface plasmon resonance (SPR) sensing and hyperspectral fluorescence analysis in this paper. A symmetrical metal-dielectric slab scheme is employed for the excitation of coupled plasmon waveguide resonance (CPWR) in the present work. Resonance between surface plasmon mode and the guided waveguide mode generates narrower full width half-maximum of the reflective curves which leads to increased precision for the determination of refractive index over conventional SPR sensors. In addition, CPWR also offers longer surface propagation depths and higher surface electric field strengths that enable the excitation of fluorescence with hyperspectral technique to maintain an appreciable signal-to-noise ratio. The refractive index information obtained from SPR sensing and the chemical properties obtained through hyperspectral fluorescence analysis confirm each other to exclude false-positive or false-negative cases. The sensor provides a comprehensive understanding of the biological events on the sensor chips.
Highlights
Surface plasmon resonance (SPR) sensor is the most commercially successful optical sensor[1,2] that could be utilized in a wide range ofelds from fundamental researches[3,4] to chemical,[5] biological[6] and clinical applications.[7]
The resonances between guided light modes within the waveguide layer and surface plasmon polarizations leads to narrower full width half-maximum (FWHM) of the re°ective curves and this in turn leads to increased resolution of the sensors by reducing the uncertainty of the resonance position
Our work in this paper proposes an implementation of symmetrical metal-dielectric structure
Summary
Surface plasmon resonance (SPR) sensor is the most commercially successful optical sensor[1,2] that could be utilized in a wide range ofelds from fundamental researches[3,4] to chemical,[5] biological[6] and clinical applications.[7] SPR uses an optical method to measure molecular binding events at the metal surface by recording the variation of resonance position caused by the change of refractive index (RI). Based on the SPR-generated exponential evanescenteld, SPR sensors permit the applications in chemical or biological researches with the advantage of labeling free, high sensitivity, low background disturbance and real-time analysis of dynamical reaction process.[8]. Theoretical results indicate that CPWR results in better performance no matter for detection range or for enhanced electromagneticeld[19] which is highly suitable for background excitation of °uorescence to maintain an appreciable signal-to-noise ratio
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