Abstract
In this paper the internal contactor is a newly developed device for the primary recovery of protein from crude feedstock. Ion exchanges (DEAE-Streamline) are confined inside the internal contactor in a stirred tank. Interactions between the ion exchange in the internal contactor and protein (BSA) in yeast suspension have been studied. For better performance, two strategies are considered: to determine the ion conductivity of a simulated yeast suspension, and to select the optimum process time for adsorption. In this system, advantages of both batch adsorption and expanded bed adsorption were obtained. Furthermore, in denser cell concentration (50 g/L) where EBA cannot be operated, the primary recovery was carried out in 1–2 hr. The efficiency of yield is higher than 80% in this condition.
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