Abstract
Exciting progress has been made recently in biophysical techniques that allow optical manipulation and measurement for single molecules at subnanometer scale as they undergo conformational transitions in real time. However, a serious problem that could limit the future application of this technique is the photo damage to biological molecules brought by intense laser irradiation. The state-of-the-art high resolution optical tweezers instrument utilizes lasers that operate at 1064 nm, which coincides with the absorption of molecular oxygen in water.
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