Abstract
Abstract Biological and chemical analyses were carried out on several digesters operated on laboratory, pilot and full-scale. These digesters received a variety of substrates which included raw sewage sludge, industrial effluents and a synthetic substrate. Hexose monophosphate shunt enzymes could only be demonstrated in very active digesters receiving substrates high in carbohydrate material. The glycolytic pathway, glyoxylic acid and citric acid cycles were found in all the anaerobic digesters analysed. Enzyme activity tests revealed different stages of adaptation of a raw sewage digester adapted to a synthetic substrate. Digesters receiving different substrates showed characteristic enzyme activity patterns, but chemical analysis such as pH, alkalinity and volatile fatty acid content did not demonstrate any obvious differences between these digesters.
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