Abstract
A sensitive and specific bioautographic method for detecting choline and its derivatives has been developed, utilizing a Neurospora crassa mutant. As little as 0.03 μg of choline chloride is detectable. The applicability of the method for detection of choline derivatives in lipid hydrolyzates is discussed. A method for determining the rate of diffusion of compounds from paper chromatograms into bioautograph agar is described.
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