Abstract
Yeast surface displaying lipases are promising biocatalysts for biodiesel synthesis. Thermomyces lanuginosus lipase (TLL) was displayed on Pichia pastoris cell surface by screening proper anchor system, resulting in a whole-cell biocatalyst (termed D-TLL). The lipase activity of D-TLL is the highest activity to date for surface-displayed TLL, reaching 2274.7 U/g dry cell weight (DCW). The obtained biocatalyst was efficient in converting Jatropha curcas oil into biodiesel. The biodiesel yield peaked 92.6% after 12 h adopting only 3.3% (w/w) of D-TLL in the solvent-free system. As compared to free TLL, the whole-cell biocatalyst showed a dramatic reduction of 48 h in reaction time. The further treatment with betaine enabled enhanced thermostability, hydrophobicity and methanol tolerance of biocatalyst (named D-TLL-betaine), and thereby increasing the reusability of the whole-cell biocatalyst. These results exhibited that the new biocatalyst, D-TLL-betaine, has great potential for biodiesel production.
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