Abstract
Nitroreductase (NTR) plays a vital part in the activation of drugs and the biodegradation of nitroaromatic compounds. With the enhanced expression level of NTR in hypoxic solid tumors, it has been widely used as one of the biomarkers to evaluate the degree of tumor hypoxia. In this work, we reported a benzoindole-based fluorescent probe NFP-NTR for nitroreductase detection. NFP-NTR can react with NTR specifically to cleave the p-nitrobenzyl ether bond when nicotinamide adenine dinucleotide (NADH) is present, showing significant fluorescence augment characteristics. Encouragingly, NFP-NTR exhibited excellent selectivity and satisfactory sensitivity, with a detection limit of 17 ng/mL. In addition, this probe has been successfully used to image live Hela cells under hypoxic conditions with low cytotoxicity, indicating that probe NFP-NTR has great potential to further elucidate more biological applications of NTR.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Photochemistry and Photobiology A: Chemistry
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
