Abstract
We demonstrated controlled conversion of DNA/Ag NCs between bright and dark states and designed a new molecular probe, NCB, for homogeneous detection of nucleic acid targets. Not relying on Forster energy transfer as the fluorescence on/off switching mechanism, NCBs have the potential to reach an ultrahigh S/B ratio in molecular sensing. Since the fluorescence enhancement is caused by intrinsic nucleobases, our detection technique is simple, inexpensive, and compatible with commercial DNA synthesizers. Here, we demonstrated that a palette of NCB light-up colors can be produced from the same origin by employing different proximal sequences. We also showed that the NC-nucleation sequences capable of achieving strong red fluorescence enhancement share a common 5'-C <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> NNNNNC <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> motif.
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