Abstract
The effect of B. danysz on the Ehrlich mouse carcinoma was described in a previous report (1936). Hyperemia and other changes, which were interpreted as being of a regressive nature, were observed in the tumors following intratumoral injection of this highly pathogenic microorganism. The experiments to be described in this paper deal with the attempt to immunize mice against infection with B. danysz by inoculation of a vaccine of that organism, the study of tumor transplants in these animals, and the reaction of the animals as well as the tumors to the injection of virulent B. danysz as compared to that of immunized but non-tumor-bearing animals. The effect of intraperitoneal injections of toxin and heat-killed cultures of B. danysz on the Ehrlich mouse carcinoma was also studied. Torrey and Kahn (1927), during their studies of the effect of proteolytic anaerobes on tumors, attempted to immunize rats actively and passively against B. histolyticus . They then noted the effect of this microorganism on the Flexner-Jobling carcinoma which had been transplanted in these animals. The immunization had no effect on tumor growth, but subsequent injection of cultures of B. histolyticus directly into the tumor apparently caused regression in a number of instances. However, this procedure was not more effective than the injection of B. histolyticus into the tumors of non-treated animals. Lewin (1928) reported that non-specific protein therapy increased the resistance of animals to tumors, for transplants grew poorly in rats and mice that had been subjected to such treatment previous to transplantation. Uhlenhuth (1937), however, found that non-specific protein therapy had no effect on the course of transplanted tumors in mice or rats.
Highlights
PRODUCTS ON TUMORSThe effect of intraperitoneal injections of toxin and heat-killed cultures of B. danysz on the Ehrlich mouse carcinoma was studied
Some proliferating tumor tissue always remained after the injection of B. danysz, so that it can be said that the regressive changes which may ensue after an injection of a virulent strain of this organism never affect the entire tumor
A relative or absolute immunity against infection by B. danysz can be produced in mice by the subcutaneous injection of a vaccine of this microorganism
Summary
The effect of intraperitoneal injections of toxin and heat-killed cultures of B. danysz on the Ehrlich mouse carcinoma was studied. Torrey and Kahn (1927), during their studies of the effect of proteolytic anaerobes on tumors, attempted to immunize rats actively and passively against B. histolyticus They noted the effect of this microorganism on the Flexner-Jobling carcinoma which had been transplanted in these animals. The immunization had no effect on tumor growth, but subsequent injection of cultures of B. histolyticus directly into the tumor apparently caused regression in a number of instances. When the tumors were between ten and fourteen days in development, 0.1 c.c. of a saline suspension of living B. danysz, prepared as previously described from an agar-slant culture, was injected into the tumors. The mortality in the immunized animals was as follows: Day of death
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