Abstract
Human noroviruses (HuNoVs) are the dominant cause of food-borne outbreaks of acute gastroenteritis. However, fundamental researches on HuNoVs, such as identification of viral receptors have been limited by the currently immature system to culture HuNoVs and the lack of efficient small animal models. Previously, we demonstrated that the recombinant protruding domain (P domain) of HuNoVs capsid proteins were successfully anchored on the surface of Escherichia coli BL21 cells after the bacteria were transformed with a plasmid expressing HuNoVs P protein fused with bacterial transmembrane anchor protein. The cell-surface-displayed P proteins could specifically recognize and bind to histo-blood group antigens (HBGAs, receptors of HuNoVs). In this study, an upgraded bacterial surface displayed system was developed as a new platform to discover candidate receptors of HuNoVs. A thrombin-susceptible “linker” sequence was added between the sequences of bacterial transmembrane anchor protein and P domain of HuNoV (GII.4) capsid protein in a plasmid that displays the functional P proteins on the surface of bacteria. In this new system, the surface-displayed HuNoV P proteins could be released by thrombin treatment. The released P proteins self-assembled into small particles, which were visualized by electron microscopy. The bacteria with the surface-displayed P proteins were incubated with pig stomach mucin which contained HBGAs. The bacteria-HuNoV P proteins-HBGAs complex could be collected by low speed centrifugation. The HuNoV P proteins-HBGAs complex was then separated from the recombinant bacterial surface by thrombin treatment. The released viral receptor was confirmed by using the monoclonal antibody against type A HBGA. It demonstrated that the new system was able to capture and easily isolate receptors of HuNoVs. This new strategy provides an alternative, easier approach for isolating unknown receptors/ligands of HuNoVs from different samples including mammalian cell lines, oysters, and fresh produce.
Highlights
Noroviruses (NoVs) are non-enveloped, single-stranded, positive-sense RNA viruses in the Caliciviridae family (Jiang et al, 1993)
We have previously reported that Human noroviruses (HuNoVs) VP1 and P proteins can be displayed on the surface of Escherichia coli by appending its sequence to the N-terminal domain sequence of bacterial ice-nucleation protein (INP) (Niu et al, 2015)
We found that the thrombin-released P proteins did not exhibit a significant background of bacterial proteins (Figure 2A)
Summary
Noroviruses (NoVs) are non-enveloped, single-stranded, positive-sense RNA viruses in the Caliciviridae family (Jiang et al, 1993). NoVs have been sub-divided into seven genogroups (GI-GVII), based on the genomic sequence of its major capsid protein (VP1). NoV GI, GII, and GIV are capable of infecting humans, comprising the human noroviruses (HuNoVs) (Vinje, 2015). HuNoVs are the main cause of human non-bacterial gastroenteritis worldwide (Hoa Tran et al, 2013). In the United States, it is estimated that 59% foodborne illnesses were caused by HuNoVs each year (Scallan et al, 2011). Of the confirmed norovirus outbreaks, 86% cases were caused by HuNoV GII strains during 2009–2012 in United States (Hall et al, 2014)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
