Abstract
A STRAIN belonging to the group Alcaligenes-Pseudomonas (referred to in the text as AP) was first isolated from a preparation of Rous virus (containing streptomycin and penicillin) obtained from a sarcoma in one of a breed of White Leghorn chickens in which the Rous sarcoma was being maintained at the Department of Zoology, University of Malaya. It had been noted that when similar preparations of virus were inoculated on the chorioallantoic membrane (CAM) of chick embryos, of the same breed, for purposes of assay, some of the embryos died in 3–6 days and had a haemorrhagic appearance. The number of deaths increased with further passages of the virus through chickens and chick embryos. The organism was recovered in an abundant pure culture, in the first instance, and similarly from embryos which died after inoculations with material containing AP or with cultures of it. The deaths of embryos in earlier…
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